{"id":5061,"date":"2024-06-13T13:05:24","date_gmt":"2024-06-13T11:05:24","guid":{"rendered":"https:\/\/remade-project.eu\/?post_type=tribe_events&#038;p=5061"},"modified":"2024-10-02T11:12:49","modified_gmt":"2024-10-02T09:12:49","slug":"hyperspectral-imaging-and-microscopy","status":"publish","type":"tribe_events","link":"https:\/\/remade-project.eu\/index.php\/event\/hyperspectral-imaging-and-microscopy\/","title":{"rendered":"Hyperspectral Imaging and microscopy"},"content":{"rendered":"<h5 style=\"text-align: center;\"><a href=\"https:\/\/ill.zoom.us\/j\/93861953973?pwd=T05rWW5WcldNdWFaaWpwa2tUQ29ZUT09\" target=\"_blank\" rel=\"noopener\">Zoom webinar<\/a> | <a href=\"https:\/\/www.youtube.com\/watch?v=ZaJLs_jZPvE\">Replay on Youtube<\/a><\/h5>\n<p>&nbsp;<\/p>\n<p><strong>Cristian Manzoni, <\/strong><br \/>\nIFN-CNR &amp; Polimi, Italy<\/p>\n<p>Spectral imaging, also known as imaging spectroscopy, refers to methods and devices for acquiring a complete light spectrum for each point in the image of a scene. It provides much richer information with respect to standard imaging, enabling to identify materials or detect dynamical processes. Spectral imaging has been applied to a wide range of scientific investigations, such as remote sensing, pigment determination in biology, medicine, coastal ocean imaging, water analysis, agriculture, cultural heritage and archaeology, just to cite a few. In particular, hyperspectral imaging aims at acquiring the whole continuous spectrum of each point of the scene. A powerful approach to this aim is to combine classical imaging with Fourier-transform spectrometry [1].<\/p>\n<figure id=\"attachment_5268\" aria-describedby=\"caption-attachment-5268\" style=\"width: 1024px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1.jpg\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-5268 size-large\" src=\"https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1-1024x255.jpg\" alt=\"\" width=\"1024\" height=\"255\" srcset=\"https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1-1024x255.jpg 1024w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1-300x75.jpg 300w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1-768x191.jpg 768w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1-1536x383.jpg 1536w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig1-2048x510.jpg 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption id=\"caption-attachment-5268\" class=\"wp-caption-text\">Hyperspectral imaging in the Thermal infrared of a sample with mineral and artificial quartz. Left: False RGB image, synthesized from the spectral data; Right: spectral emissivity.<\/figcaption><\/figure>\n<p>In this talk, I will describe the main properties of the spectral imaging and the current acquisition approaches. I will also show the most recent advancements obtained at the Istituto di Fotonica e Nanotecnologie (IFN-CNR), based on an innovative optical device [2].<\/p>\n<p>Our compact hyperspectral system is able to acquire spectral reflectance and fluorescence images with high sensitivity, broad spectral coverage and high spectral resolution. Examples of hyperspectral remote-sensing and microscopy images will be provided and discussed [3].<\/p>\n<figure id=\"attachment_5267\" aria-describedby=\"caption-attachment-5267\" style=\"width: 1024px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"size-large wp-image-5267\" src=\"https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig2-1024x319.jpg\" alt=\"\" width=\"1024\" height=\"319\" srcset=\"https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig2-1024x319.jpg 1024w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig2-300x93.jpg 300w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig2-768x239.jpg 768w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig2-1536x479.jpg 1536w, https:\/\/remade-project.eu\/wp-content\/uploads\/2024\/06\/Manzoni_Fig2-2048x638.jpg 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption id=\"caption-attachment-5267\" class=\"wp-caption-text\">Hyperspectral microscope fluorescence imaging of Invitrogen slide #1 (MitoTracker\u00ae Red CMXRos +Alexa Fluor\u00ae 488 phalloidin). Excitation: 2 lasers at 478 and 562 nm; Objective: 20X, 0.45 NA. (a) Fluorescence of Filamentous F-actin at 470-550nm; (b) Fluorescence of mitocondria at 570-680 nm; (c) Merged maps.<\/figcaption><\/figure>\n<p><em>References<\/em><\/p>\n<p><em>[1] S.P. Davis, M.C. Abrams, and J.W. Brault, Fourier Transform Spectrometry (Academic Press, 2001)<br \/>\n<\/em><em>[2] D. Brida, C. Manzoni, and G. Cerullo, \u201cPhase-locked pulses for two-dimensional spectroscopy by a birefringent delay line,\u201d Opt. Lett. 37, 3027-3029 (2012)<br \/>\n<\/em><em>[3] A. Perri, B. E. Nogueira de Faria, D. C. Teles Ferreira, D. Comelli, G. Valentini, F. Preda, D. Polli, A. M. de Paula, G. Cerullo, and C. Manzoni, \u201cHyperspectral imaging with a TWINS birefringent interferometer,\u201d Opt. Express <strong>27<\/strong>, 15956-15967 (2019)<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Zoom webinar | Replay on Youtube &nbsp; Cristian Manzoni, IFN-CNR &amp; Polimi, Italy Spectral imaging, also known as imaging spectroscopy, refers to methods and devices for acquiring a complete light 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